A7058
Sigma

Monoclonal Anti-polyHistidine−Peroxidase antibody produced in mouse

clone HIS-1, purified immunoglobulin, lyophilized powder

Synonym

Monoclonal Anti-polyHistidine

Expand/Collapse All

Descriptions

Immunogen

recombinant polyHistidine tagged fusion protein.

Preparation Note

Prepared by the two-step glutaraldehyde method described by Avrameas, S., et al., Scand. J. Immunol., 8, Suppl. 7, 7 (1978).

Reconstitution

The antibody conjugate should be reconstituted with 0.5 ml deionized water.

Specificity

The antibody recognizes synthetic polyHistidine, as well as native or denatured, reduced forms of proteins tagged with 6X histidines, expressed in selected vectors.

Physical form

Lyophilized from 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 0.01% thimerosal

Properties

antibody form

purified immunoglobulin

clone

HIS-1

form

lyophilized powder

packaging

vial of 0.5 mL

application(s)

immunoblotting: 1:2,000 using lysates of Escherichia coli induced to express a 6xHis tagged protein

isotype

IgG2a

storage temp.

2-8°C

Safety

WGK Germany

Related Categories

... Molecular Biology > Antibodies to Peptide and Protein Fusion Tags

... Clone Index > H

... Antibodies and Conjugates > Histidine/Polyhistidine

... Alphabetical Index > PO to PY

... Purification and Detection > Polyhistidine/Histidine Detection

A7058 - Datasheet (72kb)

MSDS

Certificate of Analysis

More Information

Related Categories

Printer Friendly View

Ask A Scientist

Email Page

Last 5 Products Viewed

A7058 (Sigma)