A7064
Sigma

Anti-Biotin−Alkaline Phosphatase antibody produced in goat

affinity isolated antibody, buffered aqueous glycerol solution

Synonym

Anti-Biotin

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Descriptions

Immunogen

Biotin-KLH conjugate

Specificity

Specificity and reactivity are determined by Ouchterlony Double Diffusion and immunoelectrophoresis versus the biotin-carrier protein complex. No reactivity is observed versus the carrier protein.

Physical form

Solution in 0.05 M Tris, pH 8.0, containing 10 mM glycine, 1 mM MgCl₂, 1% bovine serum albumin, 50% glycerol, and 15 mM sodium azide

Application

In some applications, localization of biotinylated probes with avidin produces high background levels. Anti-biotin reagents may be substituted for avidin to decrease non-specific binding.

Properties

antibody form

affinity isolated antibody

form

buffered aqueous glycerol solution

application(s)

direct ELISA: 1:30,000

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50

shipped in

wet ice

storage temp.

2-8°C