Adenosine 5′-Triphosphatase from porcine cerebral cortex 

This membrane enzyme hydrolyzes ATP to ADP and orthophosphate. The hydrolysis is coupled with the exchange of sodium and potassium ions across the plasma membrane. The enzyme is present in almost all tissues of higher organisms, but it is most abundant in the kidney where it reabsorbs sodium ions from the glomerular filtrate. Na⁺,K⁺-ATPase is affected by changes in intracellular calcium. It is also sensitive to ouabain and inhibited by vanadate, and (in vitro) by SPAI-1, a peptide isolated from porcine duodenum. It has been reported to be regulated by phosphorylation by protein kinase A (PKA).

Sodium iodide extracted fraction of Nakao, et al., Biochem. Biophys. Res. Commun., 19, 755 (1965).

One unit will liberate 1.0 μmole of inorganic phosphorus from ATP per min at pH 7.8 at 37 °C in the presence of Na⁺, K⁺ and Mg²⁺.

Lyophilized powder containing 90% sucrose, 0.4% EDTA Na₄ and 0.06% NaCl

Sodium and Potassium-Activated

lyophilized powder

Protein, ~10% biuret

pig ... ATP1A1(397481), ATP1B1(396898)

−20°C

Burnett, G.H. and Conklin, R.L. J. Lab. Clin. Med. 78, 779, (1971) abstract

Giotta, G.J., Native (Na⁺ + K⁺)-dependent adenosine triphosphatase has two trypsin-sensitive sites. J. Biol. Chem. 250, 5159-5164, (1975)

Lingrel, J.B., et al., Structure-function studies of the Na,K-ATPase. Kidney Int. (Suppl.) 44, S32-S39, (1994)

Lingrel, J.B., and Kuntzweiler, T., Na⁺,K⁺-ATPase. J. Biol. Chem. 269, 19659-19662, (1994)

Breier, A., et al., Ca²⁺-induced inhibition of sodium pump: noncompetitive inhibition in respect of magnesium and sodium cations. Gen. Physiol. Biophys. 17, 179-188, (1998)

Feschenko, M.S., and Sweadner, K.J., Conformation-dependent phosphorylation of Na, K-ATPase by protein kinase A and protein kinase C. J. Biol. Chem. 269, 30436-30444, (1994)

Yingst, D.R., Modulation of the Na,K-ATPase by Ca and intracellular proteins. Annu. Rev. Pharmacol. Toxicol. 50, 291-303, (1988)

Ishizuka, N., et al., Na⁺,K⁺-ATPase inhibition by an endogenous peptide, SPAI-1, isolated from porcine duodenum. Biochim. Biophys. Acta 1069, 259-266, (1991)

O3125, Ouabain octahydrate