Application
The primary purpose of all hot start PCR methods is to prevent Taq DNA polymerase activity prior to thermal cycling. Even if set-up is conducted on ice, the Taq DNA polymerase remains active and may elongate unwanted products such as misprimed or other nonspecific events. Primer-dimer interactions may also be amplified, which will reduce overall yield and efficiency.
One way to prevent unwanted amplification products is to add JumpStart Taq Antibody to the reaction. This efficient yet simple procedure takes only 10 minutes and effectively inactivates the Taq DNA polymerase until the first denaturation cycle. Upon heating to 70 °C, the antibody dissociates and full activity is restored to the Taq DNA polymerase for the remainder of the PCR. No extended heating step is required for reactivation, as for chemically inactivated Taq DNA polymerase. JumpStart Taq antibody works effectively on a variety of Taq DNA polymerases that are commercially available.
Features and Benefits
• Minimize non-specific amplification while increasing target yield & specificity
• Reduce set-up time and eliminate concerns associated with manual or wax hot-start methods.
Other Notes
Packaging
Supplied with dilution buffer.
Quantity
Two units of Taq DNA polymerase are inactivated by 1 test of JumpStart Taq Antibody.
Legal Information
JumpStart is a trademark of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co.
Purchase of this product is accompanied by a limited license for use in the Polymerase Chain Reaction (PCR) process for research purposes only and in conjunction with a thermal cycler whose use in the automated performance of the PCR process is covered by an up-front license fee, either by payment to Applied Biosystems or as purchased, i.e., and authorized thermal cycler. Antibody licensed for in vitro research use under U.S. Patent No. 5,338,671 and 5,587,287, and corresponding patents in other countries.
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