A8354
Sigma

Monoclonal Anti-β-Amyloid antibody produced in mouse

~2 mg/mL, clone NAB 228, purified immunoglobulin, buffered aqueous solution

Synonym

Anti-Aβ

MDL number

MFCD00803867

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Descriptions

Immunogen

synthetic peptide corresponding to amino acids 1-11 of human β-amyloid protein.

Specificity

The antibody recognizes human β-amyloid peptide, full-length amyloid precursor protein (APP), soluble-APP (sAPPβ' and sAPPα), C99 cleavage form, and Aβ (1-40/42), but not soluble-APP form sAPPβ.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Properties

antibody form

purified immunoglobulin

clone

NAB 228

form

buffered aqueous solution

mol wt

antigen mol wt ~110 kDa

species reactivity

human

concentration

~2 mg/mL

application(s)

immunoblotting: 2-4 μg/mL using cell extract of the human embryonal carcinoma NTERA-2 (NT2/D1) cells, treated for 2-3 weeks with 10 �M retinoic acid

immunohistochemistry: suitable

immunoprecipitation: suitable

indirect ELISA: suitable

microarray: suitable

isotype

IgG2a

Gene Information

human ... APP(351)

shipped in

dry ice

storage temp.

−20°C

References

Reference

Lee, E.B., et al., Secretion and intracellular generation of truncated Aβ in β-site amyloid-β precursor protein-cleaving enzyme expressing human neurons. J. Biol. Chem. 278, 4458-4466, (2003) abstract

Safety

WGK Germany

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Array

XP725, Panorama^® Antibody Microarray - XPRESS Profiler725 Kit

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