A8469
Sigma

Anti-Apaf1, N-Terminal antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

MDL number

MFCD02095847

Descriptions

Antigen Background

Apaf-1 is the mammalian equivalent of Ced-4. It forms a complex with cytochrome c and dATP that cleaves procaspase 9 to form activated caspase 9.

Immunogen

synthetic peptide corresponding to amino acids 12-28 of human Apaf1 (apoptosis protease-activating factor 1).

Physical form

Solution in phosphate buffered saline containing 0.02% sodium azide.

Properties

antibody form

affinity isolated antibody

form

buffered aqueous solution

mol wt

antigen mol wt 130 kDa

species reactivity

human, mouse

application(s)

immunoblotting: 0.5 μg/mL using a HeLa cell extract

microarray: suitable

Gene Information

human ... APAF1(317)

mouse ... Apaf1(11783)

shipped in

dry ice

storage temp.

−20°C

References

Reference

Zou, H., et al., Apaf-1, a human protein homologous to C. Cell 90, 405-413, (1997) abstract

Cecconi, F., et al., Apaf1 (CED-4 homolog) regulates programmed cell death in mammalian development. Cell 94, 727-737, (1998) abstract

Kuida, K., Caspase-9. Int. J. Biochem. Cell Biol. 32, 121-124, (2000) abstract

Safety

WGK Germany

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Related Categories

... Alphabetical Index > AM-AZ

... Antibodies for Apoptosis > Antibodies to Mitochondrial Proteins

... Antibodies to Cell and Organelle Proteins > Antibodies to Mitochondrial Proteins

A8469 - Datasheet (56kb)

MSDS

Certificate of Analysis

More Information