A8592
Sigma

Monoclonal ANTI-FLAG^® M2-Peroxidase (HRP) antibody produced in mouse

purified immunoglobulin, buffered aqueous glycerol solution

Synonym

Monoclonal ANTI-FLAG^® M2 antibody produced in mouse

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Descriptions

Frequently Asked Questions

Live Chat and Frequently Asked Questions are available for this Product.

Application

For simple, one-step detection by immunocytochemistry, ELISA, or Western blotting. Especially useful in detection of FLAG fusion proteins expressed in murine host, where secondary anti-mouse antibodies may cause cross-reactivity.

Other Notes

To view FLAG^® technology literature, visit www.sigma-aldrich.com/flagliterature.

Specificity

Binds to the FLAG epitope wherever it is located in the fusion protein: amino-terminal, Met-amino-terminal, carboxy-terminal, or internal. Binding is not Ca²⁺-dependent.

Physical form

Solution in phosphate buffered saline containing 50% glycerol plus preservative and stabilizer

Legal Information

ANTI-FLAG is a registered trademark of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co.

FLAG is a registered trademark of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co.

Properties

antibody form

purified immunoglobulin

clone

form

buffered aqueous glycerol solution

application(s)

direct ELISA: 1:20,000

isotype

IgG1

shipped in

wet ice

storage temp.

−20°C

Safety

WGK Germany

Related product

P8170, Protein G−Peroxidase from Streptococcus sp.

F1804, Monoclonal ANTI-FLAG^® M2 antibody produced in mouse

Related Categories

... Molecular Biology > Antibodies to Peptide and Protein Fusion Tags

... Alphabetical Index > F

... Antibodies and Conjugates > FLAG

... FLAG System > FLAG Antibody Conjugates

... Glycan Labeling > FLAG^® Antibodies for Detection of FLAG-labeled Glycans

... Clone Index > M

... Antibody Conjugates > Peroxidase Conjugates

A8592 - Datasheet (50kb)

Proteomics Prod Guide (140kb)

MSDS

Certificate of Analysis

More Information

Monoclonal ANTI-FLAG® M2-Peroxidase (HRP) antibody produced in mouse

purified immunoglobulin, buffered aqueous glycerol solution

Monoclonal ANTI-FLAG^® M2-Peroxidase (HRP) antibody produced in mouse