| A8721 Sigma |
Angiogenesis Protocols |
General description
Although angiogenesis-formation of new blood vessels from pre-existing ones-has emerged as a target for the treatment of many disorders, fundamental aspects remain poorly understood. To address those needs, this book is a collection of key techniques for assessing the formation of new blood vessels in both whole tissue and cell culture. The methods range from low-cost in vitro assays to cutting-edge in vivo models. Included are details of the associated techniques for the isolation, identification, and culture of microvessel endothelial cells from human adipose tissues, and for the transfection and transduction of primary human endothelial tissues.
Table of Contents
I. Special Review Article.
Therapeutic Inhibition of Angiogenesis,
II. Angiogenesis Protocols In Vivo.
Microscopic Assessment of Angiogenesis in Tumors,
In Vivo Matrigel Migration and Angiogenesis Assays,
Alginate Microbead Release Assay of Angiogenesis,
Disc Angiogenesis Assay,
Sponge Implant Model of Angiogenesis,
Hollow Fiber Assay for Tumor Angiogenesis,.
Dorsal Skinfold Chamber Preparation in Mice: Studying Angiogenesis by Intravital Microscopy,
Angiogenesis Assays Using Chick Chorioallantoic Membrane, .
Corneal Assay for Angiogenesis,
III. Angiogenesis Protocols In Vitro.
Collagen Gel Assay for Angiogenesis: Induction of Endothelial Cell Sprouting,
Chemotaxis and Chemokinesis in 3D Macromolecular Matrices: Relevance to Angiogenesis, .
Rat Aortic Ring: 3D Model of Angiogenesis In Vitro,
In Vitro Matrigel Angiogenesis Assays,
IV. Associated Techniques.
Microvessel Endothelial Cells from Human Adipose Tissues: Isolation, Identification, and Culture,
Transfection and Transduction of Primary Human Endothelial Cells,
Vascular Smooth Muscle Cells: Isolation, Culture, and Characterization,
Bovine Retinal Microvascular Pericytes: Isolation, Propagation, and Identification,
Index.
Therapeutic Inhibition of Angiogenesis,
II. Angiogenesis Protocols In Vivo.
Microscopic Assessment of Angiogenesis in Tumors,
In Vivo Matrigel Migration and Angiogenesis Assays,
Alginate Microbead Release Assay of Angiogenesis,
Disc Angiogenesis Assay,
Sponge Implant Model of Angiogenesis,
Hollow Fiber Assay for Tumor Angiogenesis,.
Dorsal Skinfold Chamber Preparation in Mice: Studying Angiogenesis by Intravital Microscopy,
Angiogenesis Assays Using Chick Chorioallantoic Membrane, .
Corneal Assay for Angiogenesis,
III. Angiogenesis Protocols In Vitro.
Collagen Gel Assay for Angiogenesis: Induction of Endothelial Cell Sprouting,
Chemotaxis and Chemokinesis in 3D Macromolecular Matrices: Relevance to Angiogenesis, .
Rat Aortic Ring: 3D Model of Angiogenesis In Vitro,
In Vitro Matrigel Angiogenesis Assays,
IV. Associated Techniques.
Microvessel Endothelial Cells from Human Adipose Tissues: Isolation, Identification, and Culture,
Transfection and Transduction of Primary Human Endothelial Cells,
Vascular Smooth Muscle Cells: Isolation, Culture, and Characterization,
Bovine Retinal Microvascular Pericytes: Isolation, Propagation, and Identification,
Index.
Related Information
Links
Page Options| |
|
© 2008 Sigma-Aldrich Co. Reproduction forbidden without permission. |