A8729
Sigma

Akt1 Active human

aqueous glycerol solution, recombinant, expressed in Escherichia coli, protein apparent mol wt ~80 kDa, ≥85% (SDS-PAGE)

Synonym

PKB α

Protein Kinase B-α

MDL number

MFCD02685868

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Descriptions

Application

Can be used in kinase activity assays, which measure the molar amount of phosphate incorporated into the Akt/SGK substrate peptide (RPRAATF) per minute per mg protein at 30�°C using a final concentration of 50 μM [³²P]-ATP.

Biochem/physiol Actions

Akt1 is an isoform of multifunctional Akt kinase, which upon activation becomes involved in glucose metabolism, transcription, survival, cell proliferation, angiogenesis, cell motility and a number of metabolic effects of insulin.

Other Notes

GST-tagged human recombinant full length Akt1

Physical form

Supplied as a solution at 15 μg in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM dithiothreitol (DTT), 0.1 mM EGTA and 30% glycerol.

Properties

recombinant

expressed in Escherichia coli

assay

≥85% (SDS-PAGE)

form

aqueous glycerol solution

mol wt