Application
For simple, one-step detection by immunocytochemistry. Especially useful in detection of FLAG fusion proteins expressed in murine host, where secondary anti-mouse antibodies might cause cross-reactivity.
Other Notes
Specificity
Binds to the FLAG epitope wherever it is located in the fusion protein: amino-terminal, Met-amino-terminal, carboxy-terminal, or internal. Binding is not Ca2+-dependent.
Physical form
Solution in phosphate buffered saline plus 1% BSA and preservative
Legal Information
ANTI-FLAG is a registered trademark of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co.
Cy is distributed under license from Amersham Biosciences Limited.
Cy3 is a trademark of GE Healthcare.
FLAG is a registered trademark of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co.