Ammonia Assay Kit 

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This kit is for the quantitative, enzymatic determination of ammonia in food and biological samples. Ammonia reacts with α-ketoglutaric acid (KGA) and reduced nicotinamide adenine dinucleotide phosphate (NADPH) in the presence of L-glutamate dehydrogenase (GlDH) to form L-glutamate and oxidized nicotinamide adenine dinucleotide phosphate (NADP⁺). The decrease in absorbance at 340 nm, due to the oxidation of NADPH, is proportional to the ammonia concentration. L-Glutamate dehydrogenase reacts specifically with ammonia. The Ammonia Assay Kit may be used to determine ammonia concentrations in the range of 0.02-15 μg/ml.

sufficient for 100 assays

2-8°C

Bergmeyer, H.U., and Beutler, H-O. Meth. Enzym. Anal. 3rd ed., New York, NY , (1985), 454-461

Neeley, W.E., and Phillipson, J., Automated Enzymatic Method for Determining Ammonia in Plasma. Clin. Chem. 34, 1868-1869, (1988) abstract

Ammonia Assay Reagent 10 vials

L-Glutamate Dehydrogenase for Ammonia Assay 1 vial

Ammonia Standard Solution 1 vial