DNase I 

Live Chat and Frequently Asked Questions are available for this Product.

• Suitable for the elimination of DNA from RNA preparations prior to sensitive applications such as RT-PCR
• Minimal RNase activity available
• Optimized 10× reaction buffer and Stop Solution for complete inactivation of DNase I

One unit completely digests 1 μg of plasmid DNA to oligonucleotides in 10 min. at 37 °C.

Because PCR can detect even a single molecule of DNA, RNA samples should be digested with DNase I before RT-PCR, and parallel reactions should be run without reverse transcriptase to check for amplification of contaminating DNA. DNase I digests double- and single-stranded DNA into oligo- and mononucleotides. Using the Reaction Buffer provided, DNA is removed from RNA preparations in a 15 minute digestion at room temperature. The DNase I is then inactivated by heating with the Stop Solution. Heating also denatures hairpins in the RNA, so the RNA can be used directly in reverse transcription.

Many commercial DNase I formulations are contaminated with residual RNases. This RNase contamination can destroy or degrade valuable RNA samples prior to reverse transcription. Laboratory comparisons have shown that Sigma's Amplification Grade DNase I demonstrates lower RNase activity than that from several leading molecular biology product suppliers (Fig. 1).

Purchase of this product is accompanied by a limited license for use in the Polymerase Chain Reaction (PCR) process for research purposes only and in conjunction with a thermal cycler whose use in the automated performance of the PCR process is covered by an up-front license fee, either by payment to Applied Biosystems or as purchased, i.e., and authorized thermal cycler.

wet ice

−20°C

D6518, Diethylenetriaminepentaacetic acid

E4378, Ethylene glycol-bis(2-aminoethylether)-N,N,N′,N′-tetraacetic acid

P9375, 1,10-Phenanthroline monohydrate

N9653, Netropsin dihydrochloride

ED2SS, Ethylenediaminetetraacetic acid disodium salt dihydrate

R5277, RT-PCR Protocols