Alkaline Phosphatase Detection Kit, Fluorescence 

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The assay used is fluorometric and therefore, is 10-100 times more sensitive than the colorimetric measurement. It is linear over a wide range of enzyme concentrations, which makes it particularly well suited for comparative analysis.
The reporter gene, encoding for alkaline-phosphatase, offers great advantages:
• The enzyme is highly stable.
• The assay is safe and easy to use. It provides rapid and reproducible results.
• When using the mutated version of the human placental alkaline phosphatase (SEAP) which is secreted out of the cells, enzyme activity in the same cell sample can be measured nondestructively and repeatedly over time using an aliquot of the culture medium. This saves the time required for cell extract preparations.

The kit contains buffers, substrate, and control enzyme for an easy and rapid alkaline-phosphatase reporter gene activity assay.

1 kit sufficient for 300 reactions (200 μl reactions)

wet ice

−20°C

4-Methylumbelliferyl phosphate disodium salt M8168

Fluorescent assay buffer

Dilution Buffer

Control enzyme