Annexin V-Cy3^™ Apoptosis Detection Kit 

Detects apoptosis earlier in the process than DNA-based assays such as TUNEL
• Rapid labeling of cells. Cell staining takes only 10 minutes.
• No cell fixation or processing required, reducing the detection time and allowing the cells to be used for further study.
• 6-Carboxyfluorescein diacetate secondary dye is included with the kit to differentiate apoptotic cells from viable and necrotic cells.

Sigma's Annexin V-Cy3 kit allows detection of annexin V bound to apoptotic cells by fluorescence microscopy. The Annexin V-Cy3 kit uses the dye Cy3.18 as the fluorochrome conjugated with annexin V. By microscopy, Cy3.18 fluoresces more brightly than the FITC conjugate. The kit includes the non-fluorescent compound 6-carboxyfluorescein diacetate (6-CFDA), which enters the cell and is hydrolyzed by the esterases present in living cells to the fluorescent compound 6-carboxyfluorescein, indicating that the cells are viable. This combination allows the differentiation among early apoptotic cells (annexin V positive, 6-CFDA positive), necrotic cells (annexin V positive, 6-CFDA negative), and viable cells (annexin V negative, 6-CFDA positive).

Cy3 is a trademark of GE Healthcare.

sufficient for 200 tests

wet ice

2-8°C

Pigault, C., et al., Formation of two-dimensional arrays of annexin V on phosphatidylserine-containing liposomes. J. Mol. Biol. 236, 199-208, (1994) abstract

Kuypers, F.A., et al., Detection of altered membrane phospholipid asymmetry in subpopulations of human red blood cells using fluorescently labeled annexin V. Blood 87, 1179-1187, (1996) abstract

Koopman, G., et al., Annexin V for flow cytometric detection of phosphatidylserine expression on B cells undergoing apoptosis. Blood 84, 1415-1420, (1994) abstract

Dachary-Prigent, J., et al., Calcium involvement in aminophospholipid exposure and microparticle formation during platelet activation: a study using Ca²⁺-ATPase inhibitors. Biochemistry 34, 11625-11634, (1995) abstract

10× Binding buffer 20 mL

6-Carboxyfluorescein diacetate 10 mg C5041

Annexin V-Cy3.18 Conjugate from human placenta 10 μg A4963