Annexin V-FITC Apoptosis Detection Kit 

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Detects apoptosis earlier in the process than DNA-based assays such as TUNEL.
• Rapid labeling of cells. Cell staining takes only 10 minutes.
• No cell fixation or processing required, reducing the detection time and allowing the cells to be used for further study.
• Propidium iodide secondary dye is included with the kit to differentiate apoptotic cells from viable and necrotic cells.

Annexin V-FITC kit allows fluorescent detection of annexin V bound to apoptotic cells and quantitative determination by flow cytometry. The AnnexinV-FITC kit uses annexin V conjugated with fluorescein isothiocyante (FITC) to label phosphatidylserine sites on the membrane surface. The kit includes propidium iodide (PI) to label the cellular DNA in necrotic cells where the cell membrane has been totally compromised. This combination allows the differentiation among early apoptotic cells (annexin V positive, PI negative), necrotic cells (annexin V positive, PI positive), and viable cells (annexin V negative, PI negative).

wet ice

2-8°C

Pigault, C., et al., Formation of two-dimensional arrays of annexin V on phosphatidylserine-containing liposomes. J. Mol. Biol. 236, 199-208, (1994) abstract

Koopman, G., et al., Annexin V for flow cytometric detection of phosphatidylserine expression on B cells undergoing apoptosis. Blood 84, 1415-1420, (1994) abstract

Dachary-Prigent, J., et al., Calcium involvement in aminophospholipid exposure and microparticle formation during platelet activation: a study using Ca²⁺-ATPase inhibitors. Biochemistry 34, 11625-11634, (1995) abstract

Kuypers, F.A., et al., Detection of altered membrane phospholipid asymmetry in subpopulations of human red blood cells using fluorescently labeled annexin V. Blood 87, 1179-1187, (1996) abstract

10× Binding buffer

AnnexinV-FITC solution A9210

Propidium Iodide solution