Betaine solution 

The addition of betaine at a final concentration of 0.8-1.6M improves the amplification of DNA by reducing the formation of secondary structure in GC-rich regions. The addition of betaine has been reported to enhance the specificity of the polymerase chain reaction by eliminating the base pair composition dependence of DNA melting.¹

The addition of betaine has been reported to enhance the specificity of the polymerase chain reaction by eliminating the base pair composition dependence of DNA melting (Rees, WA et al).

The purchase of this product does not include a license to practice the claims of Patent Nos. US 5,545,539, US 6,270,962, DE4411588, or DE4411594. The practice of the claims of these patents may require a license from the patent owners.

PCR Reagent

vial of 1.5 mL

5 M

DNase, RNase, none detected

2-8°C

1. Rees, W.A., et al., Betaine can eliminate the base pair composition dependence of DNA melting. Biochemistry 32, 137-144, (1993) abstract

Henke, W., et al., Betaine improves the PCR amplification of GC-rich DNA sequences. Nucleic Acids Res. 25, 3957-3958, (1997) abstract

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