Monoclonal Anti-Rat Kappa & Lambda Light Chains−Biotin antibody produced in mouse 

The product recognizes epitopes located on the rat κ and λ light chains on the various rat immunoglobulin classes and subclasses. The epitope recognized on the κ light chains is shared by both the 1a and 1b allotypes of this molecule. The antibody detects the κ and λ light chains derived from normal serum immunoglobulins or from myeloma proteins. Weak cross-reaction is observed with guinea pig immunoglobulins but not with human, bovine, cat, chicken, dog, goat, horse, mouse, pig, rabbit or sheep immunoglobulins.

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide

The product is a mixture derived from hybridomas RT-39 and RL-6, secreting monoclonal antibodies against rat κ and λ light chains, respectively.

purified immunoglobulin

RT-39/RL-6

buffered aqueous solution

antigen mol wt 25 kDa

direct ELISA: 1:40,000

dot blot: 1:4,000

immunoblotting (chemiluminescent): 1:40,000

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:600

IgG1/IgG2a

dry ice

−20°C

3