B1809
Sigma

Monoclonal Anti-Bcl-2α antibody produced in mouse

~0.2 mg/mL, clone 10C4, purified immunoglobulin, buffered aqueous solution

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MDL number

MFCD04040935

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Descriptions

Immunogen

synthetic peptide corresponding to amino acids 61-76 of mouse Bcl-2α.

Specificity

Does not crossreact with Bcl-x or Bax protein.

Physical form

Solution in phosphate buffered saline, pH 7.4.containing 0.2% bovine serum albumin and 15 mM sodium azide.

Properties

antibody form

purified immunoglobulin

clone

10C4

form

buffered aqueous solution

mol wt

antigen mol wt 25-26 kDa

species reactivity

mouse, rat

does not react with

human

concentration

~0.2 mg/mL

application(s)

immunoblotting: suitable

immunohistochemistry: 2-4 μg/mL using mouse or rat thymocytes, splenocytes, tonsil, or follicular lymphomas

immunoprecipitation: 10 μL

isotype

IgG1

Gene Information

mouse ... Bcl2(12043)

rat ... Bcl2(24224)

shipped in

wet ice

storage temp.

−20°C

References

Reference

Hsu, Y-T and Youle, R.J. , Nonionic Detergents Induce Dimerization among Members of the Bcl-2 Family. J. Biol. Chem. 272, 13829-13874 , (1997)

Safety

WGK Germany

NWG

B1809 - Datasheet (27kb)

Celltransmissions 19(4) (130kb)

MSDS

Certificate of Analysis

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B1809 (Sigma)

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