Immunogen
Biotin-KLH conjugate
Reconstitution
Reconstitute with 0.135 M sodium chloride.
Specificity
Specificity and reactivity are determined by Ouchterlony Double Diffusion and immunoelectrophoresis versus the biotin-carrier protein complex. No reactivity is observed versus the carrier protein.
Physical form
Lyophilized from 0.01 M sodium phosphate, 15 mM sodium chloride, pH 7.2
Application
In some applications, localization of biotinylated probes with avidin produces high background levels. Anti-biotin reagents may be substituted for avidin to decrease non-specific binding.