(Partial) 1.0 Intro 1.1 The Manner in Which the Bioproduct Is Associated With the Cell or Organism Defines Its Initial Recovery Characteristics. 1.2 Physical Processing and Water Removal Steps Are Important Separation Methods for Large Volume Products. 1.3 ...........1.8. Sedimentation, Centrifugation, and Filtration. 2.0 Introduction. 2.1 Solid/Liquid Separations by Sedimentation or Centrifugation are Based on Differences in Particle Size and Density. 2.2 Centrifugation Uses Mechanical Force to Amplify the Differences in Size and Density Between Wet Biological Materials and the Aqueous Media In Which the Solids are Found. 2.3.................2.15. Membrane Separations. 3.0 Introduction. 3.1 Microfiltration Membranes Remove Particles Whose Sizes Range from 0.1 to 10 Microns. 3.2 Molecular Filtration by Ultrafiltration and Reverse Osmosis Utilizes Supported Membranes with Nanometer Sized Pores. 3.3 ..............3.17 Precipitation Crystallization, and Extraction. 4.0 Introduction. 4.1 Addition of Neutral Salts, or An Acid or Base to Aqueous Solutions Induces the Solute to Precipitate. 4.2 Alcohols Decrease Solvating Power of Water by Lowering the Dielectric Constant of the Solution. 4.3 ..............4.28. Principles of Liquid Chromatography. 5.0 Introduction. 5.1 Liquid Chromatography Systems Are Classified by Pressures That Characterize Their Operation (HPLC, LPLC, and MPLC). 5.2 This Chapter Presents The Principles And Practices of Analyzing and Scaling-up Chromatography Column Performance from Experimental Measurements. 5.3 ............... 5.21. Liquid Chromatography Scale-up. 6.0 Introduction. Linear Chromatography. 6.1 Scale-up Rules Enable Initial Specification of Chromatography Columns. 6.2 Scale-up Rules for Size Exclusion Chromatography (SEC) Assume Pore Diffusion Controls. 6.3..............6.36. Principles of Gradient Elution Chromatography. 7.0 Introduction. 7.1 The System for Carrying Out Gradient Chromatography Is Similar to That For Isocratic Chromatography. Ion Exchange Gradient Chromatography. 7.2 Linear Gradient Elution In Ion Exchange Chromatography Is Based on Exchange of A Multivalent Protein for a Mono- or Di-valent Salt. 7.3................7.19. Summary and Perspectives. Principles of Bioseparations for Biopharmaceuticals and Recombinant Protein Products. 8.0 Introduction. 8.1 New Biotechnology Products Are the Fastest Growing Area in Bioseparations. 8.2 Bioseparation Processes Have a Significant Impact on Manufacturing Costs. 8.3 ..............8.63. Affinity Chromatography: Bridge Between Molecular Biology, Combinatorial Methods and Separations Science. 9.0 Introduction. Affinity Ligands From Combinatorial Libraries. 9.1 Combinatorial Chemistry Creates Libraries of New Peptide Sequences. 9.2 Visual Identification Finds A Needle in The Haystack: One Affinity Ligand Among Thousands of Peptides (Factor IX Example). 9.3 Liquid Chromatography Using 300 mg of Beads Confirms Specificity of Factor IX Binding..............9.41.
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