| B5678 Sigma |
Basic Molecular Biology: Essential Techniques |
General description
Begins with a brief overview and then focuses on simple step-by-step protocols of the core techniques. It provides not only a theoretical outline, but also valuable advice on real-world problems and estimates on how much time is required to perform each technique.
Table of Contents
Preparation of DNA.
Preparation of RNA.
Preparation of Plasmid DNA.
DNA Analysis by Restriction Enzyme Digestion and Southern Blotting.
DNA Analysis by PCR. RNA Analysis by Northern Blotting.
RNA Analysis by RNase Protection.
RNA Analysis by RT-PCR and Differential Display.
Analysis of Protein-DNA Interactions by Gel Shift Assay.
Gene Transfection.
Appendices.
Index.
Preparation of RNA.
Preparation of Plasmid DNA.
DNA Analysis by Restriction Enzyme Digestion and Southern Blotting.
DNA Analysis by PCR. RNA Analysis by Northern Blotting.
RNA Analysis by RNase Protection.
RNA Analysis by RT-PCR and Differential Display.
Analysis of Protein-DNA Interactions by Gel Shift Assay.
Gene Transfection.
Appendices.
Index.
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