B6186
Sigma

Anti-Beclin 1 antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

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Synonym

Anti-ATG6

Anti-BECN1

Anti-Coiled-coil myosin-like BCL2-interacting protein

Anti-GT197

Anti-VPS30

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Descriptions

Immunogen

synthetic peptide corresponding to amino acids 329-345 of human beclin 1, conjugated to KLH via an N-terminal cysteine residue. The corresponding sequence is identical in rat and mouse.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Legal Information

ExtrAvidin is a registered trademark of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co.

Properties

antibody form

affinity isolated antibody

form

buffered aqueous solution

mol wt

antigen mol wt ~60 kDa

species reactivity

human, rat, mouse

application(s)

immunoblotting: 2-4 μg/mL using whole extracts of rat PC12 and mouse 3T3 cells

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 5-10 μg/mL using biotin/ExtrAvidin^®-Peroxidase staining of heat-retrieved sections of rat brain

immunoprecipitation: 1-2 μL using extract of human HEK-293T cells expressing recombinant human Beclin 1-GFP

Gene Information

mouse ... Becn1(56208)

rat ... Becn1(114558)

human ... BECN1(8678)

shipped in

dry ice

storage temp.

−20°C

Safety

WGK Germany