Bradford Reagent 

This protein assay is based on complexing of proteins with Brilliant Blue G. The protein sample is mixed with the reagent and then read at 595 nm after a short incubation at room temperature.

• The reagent is ready to use. No mixing or dilution required.
• Color development is rapid. Only a five minute incubation and then the sample is read a 595 nm.
• Reducing sugars and reducing substances along with thiols do not interfere with this reagent.
• Reagent is suitable for micro (1-10 μg/ml) and standard (50-1400 μg/ml) assays.
• Can be used in microwell plate assays.
• Inexpensive assay.

Data sheet included with each bottle.

Coomassie is a registered trademark of Imperial Chemical Industries Ltd.

Coomassie is a trademark of ICI Americas

2-8°C

Bradford, M., A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of dye-binding. Anal. Biochem. 72, 248-254, (1976) abstract

Friedenauer, D., and Berlet, H.H., Sensitivity and variability of the Bradford protein assay in the presence of detergents. Anal. Biochem. 178, 263-268, (1989) abstract

Rubin, R.W., and Warren, R.W., Quantitation of microgram amounts of protein in SDS-mercaptoethanol-tris electrophoresis sample buffer. Anal. Biochem. 83, 773-777, (1977) abstract

Splittgerber, A.G., Sohl, J., Nonlinearity in protein assays by the Coomassie blue dye-binding method. Anal. Biochem. 179, 198, (1989) abstract

Tal, M., et al., Why does Coomassie Brilliant Blue interact differently with different proteins? A partial answer. J. Biol. Chem. 260, 9976, (1985) abstract

Compton, S.J., and Jones, C.G., Mechanism of dye response and interference in the Bradford protein assay. Anal. Biochem. 151, 369-374, (1985) abstract

UN 1805 8/PG 3

1

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