| B7157 Sigma |
Baculovirus Expression Vectors: A Laboratory Manual |
General description
This laboratory manual simplifies the selection of the most appropriate vector design for a given problem and describes each step in the implementation process from vector construction to large-scale protein production.
Table of Contents
PART I: AN OVERVIEW OF BACULOVIRUSES
1. Virus Structure and the Infection Process
2. Gene Organization, Regulation, and Function
3. Virus-Host Interactions
4. Summary of Baculovirus Features Relevant to Expression Factors
PART II: CHOOSING A TRANSFER PLASMID AND PARENT VIRUS
5. Choice of Virus and Host Species
6. Choice of Transfer Plasmid
7. Available Transfer Plasmids
8. Choosing a Parent Virus for Use in Vector Construction
9. Optimizing Expression: Tailoring the Heterologous Gene to the Transfer Plasmid and the Baculovirus Expression System
PART III: METHODS FOR VECTOR CONSTRUCTION AND GENE EXPRESSION
10. Safety Considerations
11. Insect Cell Culture
12. Virus Methods
13. Contransfection and Recombinant Virus Identification
14. Characterizing Recombinant Gene Expression
15. Post-Translational Modification
PART IV: METHODS FOR SCALE-UP OF PROTEIN PRODUCTION AND USE OF INSECT LARVAE
16. Scale-Up of Protein Production in a Stirred Bioreactor, David L. Clemm
17. Scale-Up of Protein Production in an Airlift Fermenter, Cheryl Isaac Murphy
18. Insect Rearing and Infection
Appendix 1: Nucleotide Sequence of AcMNPV Polyhedrin Region
Appendix 2: Nucleotide Sequence of AcMNPV p10 Region
Appendix 3: List of Genes Expressed in the Baculovirus Expression System
Appendix 4: Suppliers
Appendix 5: Stock Solutions
Appendix 6: Excel Spreadsheet for TCID50 Calculation
References
Index
1. Virus Structure and the Infection Process
2. Gene Organization, Regulation, and Function
3. Virus-Host Interactions
4. Summary of Baculovirus Features Relevant to Expression Factors
PART II: CHOOSING A TRANSFER PLASMID AND PARENT VIRUS
5. Choice of Virus and Host Species
6. Choice of Transfer Plasmid
7. Available Transfer Plasmids
8. Choosing a Parent Virus for Use in Vector Construction
9. Optimizing Expression: Tailoring the Heterologous Gene to the Transfer Plasmid and the Baculovirus Expression System
PART III: METHODS FOR VECTOR CONSTRUCTION AND GENE EXPRESSION
10. Safety Considerations
11. Insect Cell Culture
12. Virus Methods
13. Contransfection and Recombinant Virus Identification
14. Characterizing Recombinant Gene Expression
15. Post-Translational Modification
PART IV: METHODS FOR SCALE-UP OF PROTEIN PRODUCTION AND USE OF INSECT LARVAE
16. Scale-Up of Protein Production in a Stirred Bioreactor, David L. Clemm
17. Scale-Up of Protein Production in an Airlift Fermenter, Cheryl Isaac Murphy
18. Insect Rearing and Infection
Appendix 1: Nucleotide Sequence of AcMNPV Polyhedrin Region
Appendix 2: Nucleotide Sequence of AcMNPV p10 Region
Appendix 3: List of Genes Expressed in the Baculovirus Expression System
Appendix 4: Suppliers
Appendix 5: Stock Solutions
Appendix 6: Excel Spreadsheet for TCID50 Calculation
References
Index
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