B7554
Sigma

Monoclonal Anti-c-Myc−Biotin antibody produced in mouse

clone 9E10, purified immunoglobulin, buffered aqueous solution

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Synonym

Anti-c-Myc

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Descriptions

Immunogen

synthetic peptide corresponding to residues 408-439 of the human p62^c-Myc protein.

Specificity

Specificity: N-terminal or C-terminal c-Myc (Glu-Gln-Lys-Leu-Ile-Ser-Glu-Glu-Asp-Leu) tagged fusion proteins.

Staining by immunoblotting is specifically inhibited by the c-Myc peptide (Product Code M2435).

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide as a preservative.

Properties

antibody form

purified immunoglobulin

clone

9E10

form

buffered aqueous solution

application(s)

immunoblotting (chemiluminescent): 0.05-0.1 μg/mL using an extract of 293T cells expressing c-Myc tagged fusion protein

Gene Information

human ... MYC(4609)

shipped in

dry ice

storage temp.

−20°C

References

Reference

Evan, G.I., et al., Isolation of monoclonal antibodies specific for human c-myc proto-oncogene product. Mol. Cell Biochem. 5, 3610-3616, (1985)

Campbell, A.M., et al., The alternative carboxyl termini of avian cardiac and brain sarcoplasmic reticulum/endoplasmic reticulum Ca(2+)-ATPases are on opposite sides of the membrane. J. Biol. Chem. 267, 9321-9325, (1992)

Robertson, D., et al., Ultrastructural localization of ras-related proteins using epitope-tagged plasmids. J. Histochem. Cytochem. 43, 471-480, (1995)

Royds, J.A., et al., Cellular localisation of c-myc product in human colorectal epithelial neoplasia. J. Pathol. 166, 225-233, (1992)

Williams, A.R.W., et al., Immunohistochemical demonstration of altered intracellular localization of the C-Myc oncogene product in human colorectal neoplasms. J. Pathol. 160, 287-293, (1990)

Androniki, T., et al., Implication of the ras and myc oncoproteins in the pathogenesis of mycosis fungoides. Anticancer Res. 11, 1433-1438, (1991)

Tiniakos, D., et al., Expression of ras and myc oncogenes in human hepatocellular carcinoma and non-neoplastic liver tissues. Anticancer Res. 9, 715-721, (1989)

Karayiannis, M., et al., Evaluation of the ras and myc oncoproteins in benign gastric lesions. Anticancer Res. 10, 1127-1133, (1990)

Gosney, J.R., et al., c-myc oncoprotein in bronchial carcinoma: expression in all major morphological types. Anticancer Res. 10, 623-628, (1990)

Kobayashi, H., et al., Identification of the domains in cyclin A required for binding to, and activation of, p34cdc2 and p32cdk2 protein kinase subunits. Mol. Biol. Cell 11, 1279-1294, (1992)

Hu, Q., et al., Ras-dependent induction of cellular responses by constitutively active phosphatidylinositol-3 kinase. Science 268, 100-102, (1995)

Kleymann, G., et al., Use of antibody fragments (Fv) in immunocytochemistry. J. Histochem. Cytochem. 43, 607-614, (1995)

Safety

WGK Germany