Anti-fd Bacteriophage antibody produced in rabbit 

fd bacteriophage

The antibody binds specifically to phage coat proteins of fd phage or M13 phage and thus may act as a capture antibody when coated directly on multiwell plates or as a primary detection antibody for specifically captured fd or M13 phage. The antibody may be useful in rapidly sorting large phage display libraries (antibody, peptide, etc.) with the expressed proteins fused to either the gene III or to the gene VIII protein of the filamentous phage. The antibody may be used as a reagent in "phage ELISA" offering sensitive and specific activity for detection of recombinant phages.

Solution supplied in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide

IgG fraction of antiserum

buffered aqueous solution

indirect ELISA: 1:1,000 using 5 × 10⁷ phage/ml or 5 × 10¹⁰ phage/ml, respectively

dry ice

−20°C

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