B9428
Sigma

Monoclonal Anti-Bin1 antibody produced in mouse

clone 99D, ascites fluid

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Synonym

Anti-Amphiphysin II

Anti-ampl

Anti-SH3P9

MDL number

MFCD02095896

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Descriptions

Immunogen

recombinant polypeptide containing amino acids 189-398 of human Bin1.

Specificity

The antibody reacts specifically with a 33-residue segment of the myc-binding domain (MBD, amino acids 323-356) of the human Bin1 molecule. It does not react with the Bin1-related 45 kDa polypeptide.

Properties

antibody form

ascites fluid

clone

99D

mol wt

antigen mol wt 65-75 kDa

contains

15 mM sodium azide

species reactivity

human, mouse, rat, rabbit, avian

application(s)

flow cytometry: suitable

immunoblotting: 1:50,000 using a rat brain cytosol preparation

immunocytochemistry: suitable using nucleoplasm, subnuclear punctate compartment, and cytoplasm

immunohistochemistry (frozen sections): suitable

immunoprecipitation: suitable

indirect ELISA: suitable

isotype

IgG2b

Gene Information

mouse ... Bin1(30948)

rat ... Bin1(117028)

human ... BIN1(274)

shipped in

dry ice

storage temp.

−20°C

References

Reference

Wechsler-Reya R., et al., The putative tumor suppressor BIN1 is a short-lived nuclear phosphoprotein, the localization of which is altered in malignant cells. Cancer Res. 57, 3258-3263, (1997) abstract

Wechsler-Reya, R.J., et al., A role for the putative tumor suppressor Bin1 in muscle cell differentiation. Mol. Cell. Biol. 18, 566-575, (1998) abstract

Safety

WGK Germany