BE0100
Sigma

β Amyloid 1-40 ELISA human

sufficient for 96 multiwell tests

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Synonym

β Amyloid ELISA

Amyloid β ELISA

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Descriptions

Features and Benefits

The assay is a sensitive (10 pg/mL), and specific alternative to immunoblotting or RIA.

Application

Human β-amyloid (hAβ) ELISAs are designed for in vitro quantitative detection of native and recombinant β-amyloid in sera, cell culture supernatants and other biological samples. hAβ's are main component of the protein deposits in neurofibrillary tangles, neuritic plaques and neuropil threads in the cerebral cortex of Alzheimer's Disease (AD) and Down's Syndrome. The antibodies used in these kits selectively differentiate between Aβ40 and Aβ42/Aβ43.

The ELISA can be used for in vitro quantitative determination of human native and recombinant β-amyloid 1-40 (hAβ40) protein in serum, cell culture supernatants and other biological sample with no crossreactivity with Aβ42/Aβ43.

General description

A solid-phase sandwich enzyme-linked immunosorbent assay (ELISA). A monoclonal capture antibody specific for the NH₂ terminus of hAβ has been coated onto the multiwell strips. hAβ antigen in standards and samples binds to the capture antibody. A detection antibody specific for the hAβ 1−40 or 1−42 sequence binds the immobilized hAβ. An anti-rabbit IgG-HRP completes the sandwich. The reaction is visualized by TMB substrate, followed by the stop solution. The intensity of the yellow color is directly proportional to the concentration of hAβ in the original sample.

Properties

usage

sufficient for 96 multiwell tests

shipped in

wet ice

storage temp.

2-8°C

References

Reference

Citron, M. et al., β-secretase cleavage of Alzheimer's amyloid precursor protein by the transmembrane aspartic Protease BACE. . Science 286, 735-741 , (1999)

Savage, M.J., et al., Turnover of ß-amyloid protein in mouse brain and acute reduction of its level by phorbol ester. J. Neurosci. 18, 1743-1752 , (1998)

Safety

Safety Statements

23-24/25

RIDADR

UN 3316 9/PG 3

Components

Kit component only

hAβ 1-40 Standard, Lyophilized synthetic peptide

Monoclonal Anti-hAβ NH₂-Terminus Specific Antibody-coated 96 well plate

Anti-hAβ 1-40 Antibody

Anti-Rabbit IgG-HRP Concentrate, 100X

HRP Diluent