Cyclooxygenase 2 human 

COX-2 catalyzes the conversion of arachidonic acid to prostaglandin H₂ (the first step in the biosynthesis of prostaglandins, thromboxanes, and prostacyclins). It is the inducible form of cyclooxygenase, induced by phorbol esters, lipopolysaccharides, and cytokines. COX-2 is upregulated in the neovasculature of malignant tumors. Inhibition of COX-2 with nonsteroidal anti-inflammatory drugs (NSAIDs) is associated with decreased tumor growth.

Tagged with a six-residue histidine sequence near the N-terminus.

K_M for arachidonate = 6.5 μM. Exhibits activity and sensitivity to NSAIDs similar to the non-tagged enzyme.

One unit consumes one nanomole of oxygen per minute at 37 °C in 0.1 M Tris-HCl buffer, pH 8, containing 100 μM arachidonate, 5 mM EDTA, 2 mM phenol, and 1 μM hematin.

Solution in 80 mM Tris-HCl, pH 8, with 0.1% TWEEN® 20 and 300 μM diethyldithiocarbamate.

expressed in Sf21 cells

≥70% (SDS-PAGE)

aqueous solution

homodimer subunit mol wt 70-74 kDa

human ... PTGS2(5743)

dry ice

−70°C

Xie, W., et al., Expression of a mitogen-responsive gene encoding prostaglandin synthase is regulated by mRNA splicing. Proc. Natl. Acad. Sci. USA 88, 2692, (1991)

Masferrer, J., Approach to angiogenesis inhibition based on cyclooxygenase-2. Cancer J. 7 Suppl. 3, S144-S150, (2001)

Smith, T., et al., Purification and characterization of the human recombinant histidine-tagged prostaglandin endoperoxide H synthases-1 and -2. Arch. Biochem. Biophys. 375, 195, (2000)

NWG