C1427
Sigma

Cytochrome b₅ human

recombinant, expressed in Escherichia coli (histidine-tagged), ≥90% (SDS-PAGE)

MDL number

Descriptions

Analysis Note

Heme content measured using difference spectroscopy. An extinction coefficient of 100 mM^-1 for the absorbance difference between 490 nm and 424 nm is used to calculate the concentration of cytochrome b₅.

Physical form

Solution in 20 mM phosphate buffer, pH 7.25, with 0.1 mM EDTA

Biochem/physiol Actions

Cytochrome b₅ is a membrane-bound heme-containing protein that enhances the catalytic efficiency of some P450 isoforms. Its mechanism of action is unknown.

Properties

recombinant

expressed in Escherichia coli (histidine-tagged)

assay

≥90% (SDS-PAGE)

mol wt

mol wt 16 kDa

Gene Information

human ... CYB5A(1528)

shipped in

dry ice

storage temp.

−70°C

References

Reference

Loughran, P.A. et al., The kinetic and spectral characterization of the E. coli-expressed mammalian CYP4A7: cytochrome b₅ effects vary with substrate. Arch. Biochem. Biophys. 385, 311, (2001)

Schenkman, J.B., Jansson, I., Interactions between cytochrome P450 and cytochrome b₅. Drug Metab. Rev. 31, 351, (1999)

Helvig, C., et al., Molecular, enzymatic, and regulatory characterization of rat kidney cytochromes P450 4A2 and 4A3. Biochemistry 37, 12546, (1998)

Safety

WGK Germany