| C1854 Sigma |
Chromatin Protocols |
General description
Collection of protocols is designed to analyze the relationship between chromatin structure and function, and to elucidate molecular mechanisms that control vital cellular functions such as transcription, replication, recombination, and DNA repair. Methods cover topics from cell-free systems for the constitution of chromatin heterogeneity in vitro, to techniques for in vivo analysis of protein–DNA interactions. Methods include instructions to ensure successful replication and to avoid pitfalls.
Table of Contents
Expression and Purification of Recombinant Histones and Nucleosome Reconstitution.
Preparation and Analysis of Positioned Nucleosomes,
Site-Directed Chemical Probing of Histone-DNA Interactions,
Base-Pair Resolution Mapping of Nucleosomes In Vitro.
Equilibrium and Dynamic Nucleosome Stability,
Nucleosome Structure and Dynamics: The DNA Minicircle Approach,
Analysis of Linker Histone Binding to Mono- and Dinucleosomes,
Quantitative Analysis of Chromatin Higher-Order Organization Using Agarose Gel Electrophoresis.
Analytical Ultracentrifugation of Chromatin. Analysis of Chromatin by Scanning Force Microscopy,
In Vivo Mapping of Nucleosomes Using Psoralen-DNA Crosslinking and Primer Extension.
Preparation of Chromatin Assembly Extracts from Xenopus Oocytes.
Preparation of Chromatin Assembly Extracts from Preblastoderm Drosophila Embryos,
A Solid-Phase Approach for the Analysis of Reconstituted Chromatin,
Reconstitution and Analysis of Hyperacetylated Chromatin,
Assembly of Mitotic Chromosomes in Xenopus Egg Extract,
Nucleotide Excision Repair Coupled to Chromatin Assembly,
Photolyase: A Molecular Tool To Characterize Chromatin Structure in Yeast,
Transcriptional and Structural Analyses of Isolated SV40 Chromatin,
In Vitro Replication of Chromatin Templates, Analysis of HMG-14/-17-Containing Chromatin,
Identification and Analysis of Native Nucleosomal Histone Acetyltransferase Complexes,
Analysis of Nucleosome Disruption by ATP-Driven Chromatin Remodeling Complexes,
Nucleosome Remodeling Factor NURF and In Vitro Transcription of Chromatin,
An SDS-PAGE-Based Enzyme Activity Assay for the Detection and Identification of Histone Acetyltransferases,
Analysis of DNaseI Hypersensitive Sites in Chromatin by Cleavage in Permeabilized Cells,
Mapping of Nucleosome Positions in Yeast. Analysis of DNA Topology in Yeast Chromatin,
DNA Methyltransferases as Probes for Chromatin Structure in Yeast,
Restriction Nucleases as Probes for Chromatin Structure,
Genomic Footprinting Using Nucleases,
In Situ Analysis of Chromatin Proteins During Development and Cell Differentiation Using Flow Cytometry,
Mapping DNA Target Sites of Chromatin Proteins in Vivo by Formaldehyde Crosslinking,
Mapping DNA Interaction Sites of Chromosomal Proteins: Crosslinking Studies in Yeast,
UV Laser Footprinting and Protein-DNA Crosslinking: Application to Chromatin,
An In Vivo Crosslinking Assay That Detects DNA Binding by Sequence-Specific Transcription Factors,
Index.
Preparation and Analysis of Positioned Nucleosomes,
Site-Directed Chemical Probing of Histone-DNA Interactions,
Base-Pair Resolution Mapping of Nucleosomes In Vitro.
Equilibrium and Dynamic Nucleosome Stability,
Nucleosome Structure and Dynamics: The DNA Minicircle Approach,
Analysis of Linker Histone Binding to Mono- and Dinucleosomes,
Quantitative Analysis of Chromatin Higher-Order Organization Using Agarose Gel Electrophoresis.
Analytical Ultracentrifugation of Chromatin. Analysis of Chromatin by Scanning Force Microscopy,
In Vivo Mapping of Nucleosomes Using Psoralen-DNA Crosslinking and Primer Extension.
Preparation of Chromatin Assembly Extracts from Xenopus Oocytes.
Preparation of Chromatin Assembly Extracts from Preblastoderm Drosophila Embryos,
A Solid-Phase Approach for the Analysis of Reconstituted Chromatin,
Reconstitution and Analysis of Hyperacetylated Chromatin,
Assembly of Mitotic Chromosomes in Xenopus Egg Extract,
Nucleotide Excision Repair Coupled to Chromatin Assembly,
Photolyase: A Molecular Tool To Characterize Chromatin Structure in Yeast,
Transcriptional and Structural Analyses of Isolated SV40 Chromatin,
In Vitro Replication of Chromatin Templates, Analysis of HMG-14/-17-Containing Chromatin,
Identification and Analysis of Native Nucleosomal Histone Acetyltransferase Complexes,
Analysis of Nucleosome Disruption by ATP-Driven Chromatin Remodeling Complexes,
Nucleosome Remodeling Factor NURF and In Vitro Transcription of Chromatin,
An SDS-PAGE-Based Enzyme Activity Assay for the Detection and Identification of Histone Acetyltransferases,
Analysis of DNaseI Hypersensitive Sites in Chromatin by Cleavage in Permeabilized Cells,
Mapping of Nucleosome Positions in Yeast. Analysis of DNA Topology in Yeast Chromatin,
DNA Methyltransferases as Probes for Chromatin Structure in Yeast,
Restriction Nucleases as Probes for Chromatin Structure,
Genomic Footprinting Using Nucleases,
In Situ Analysis of Chromatin Proteins During Development and Cell Differentiation Using Flow Cytometry,
Mapping DNA Target Sites of Chromatin Proteins in Vivo by Formaldehyde Crosslinking,
Mapping DNA Interaction Sites of Chromosomal Proteins: Crosslinking Studies in Yeast,
UV Laser Footprinting and Protein-DNA Crosslinking: Application to Chromatin,
An In Vivo Crosslinking Assay That Detects DNA Binding by Sequence-Specific Transcription Factors,
Index.
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