Section 1: Introduction.
Introduction to the Polymerase Chain Reaction,
SETTING UP A PCR LABORATORY,
Section 2: General Methodology.
Amplification from Archival Materials,
Quantitative PCR,
Mutation Screening Using PCR-SSCP: Silver Staining and Isotopic Protocols,
Multiple Fluorescence-Based PCR-SSCP Analysis with Primer-, Post-, and Internal Labeling,
The Amplification Refractory Mutation System,
Artificial Restriction Fragment Length Polymorphism (A-RFLP) Analysis,
Long Range PCR,
Generation of Labeled Probes by PCR,
PCR of rthe Detection of Minority DNA Populations,
In Situ Amplification,
Sequencing of PCR Products,
Reverse Transcriptase-Polymerase Chain Reaction (RT-PCR),
Section 3: Clinical Applications.
PCR Amplification of Minisatellite DNA for the Detection of Mixed Chimerism After Bone Marrow Transplantation,
Diagnosis of Mitochondrial Disorders Using the PCR,
PCR Analysis of CD-44 Variants in Tumors,
Detection of Circulating Solid Tumor Cells by Reverse Transcriptase Polymerase Chain Reaction,
Detection of Circulating Cancer Cells Using Immunobead-PCR,
Single Cell PCR: Theory, Practice, and Clinical Applications,
PCR-Based Noninvasive Prenatal Diagnosis Using Fetal Cells in Maternal Circulation,
Noninvasive Prenatal Diagnosis Using a Single Fetal Nucleated Erythrocyte Isolated by Micromanipulation from Maternal Blood,
Diagnosis of Chromosomal Anueploidies Using Quantitative Fluorescent PCR.
Detection of the Hepatitis C Virus by RT-PCR,
The Application of PCR to the Detection of M. tuberculosis in Sputum Samples,
Microsatellite Analysis in Human Disease,
Index.
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