| C2481 Sigma |
Cytokine Cell Biology: A Practical Approach |
General description
This focuses on cell biology techniques for studying cytokines, cytokine receptors, and cytokine driven processes. Assays for human B cell responses, leucocyte migration, haematopoietic growth factors, macrophage activation by cytokines, RIA, IRMA, and ELISA assays, and quantitative biological assays for cytokines are covered. There are chapters on studying cytokine regulation of endothelial cells, the measurement of proliferative, cytostatic, and cytolytic activity of cytokines, and the use of flow cytometry and intracellular fluorescent staining
Table of Contents
1. Measurement of proliferative, cytostatic, and cytolytic activity of cytokines,
2. Human B-cell responses to cytokines,
3. T cells and cytokines,
4. The generation and quantitation of cell-mediated cytotoxicity,
5. Cytokine regulation of endothelial cells,
6. Assays for leucocyte migration, J
7. Biological assays for haemopoietic growth factors,
8. Assays for macrophage activation by cytokines, .
9. Cytokine production by individual cells,
10. Flow cytometric detection of intracellular cytokines, .
11. Development of antibodies to cytokines,
12. RIA, IRMA, and ELISA for cytokines and their soluble receptors,
13. Quantitative biological assays,
A1. List of suppliers
2. Human B-cell responses to cytokines,
3. T cells and cytokines,
4. The generation and quantitation of cell-mediated cytotoxicity,
5. Cytokine regulation of endothelial cells,
6. Assays for leucocyte migration, J
7. Biological assays for haemopoietic growth factors,
8. Assays for macrophage activation by cytokines, .
9. Cytokine production by individual cells,
10. Flow cytometric detection of intracellular cytokines, .
11. Development of antibodies to cytokines,
12. RIA, IRMA, and ELISA for cytokines and their soluble receptors,
13. Quantitative biological assays,
A1. List of suppliers
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