C2832
Sigma

Cholesteryl 3β-N-(dimethylaminoethyl)carbamate hydrochloride

~95%

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Synonym

3β-[N-(Dimethylaminoethane)carbamoyl]cholesterol

DC-Chol

Molecular Formula

C₃₂H₅₇ClN₂O₂

Molecular Weight

537.26

CAS Number

166023-21-8

MDL number

MFCD00467173

PubChem Substance ID

24892536

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Descriptions

Application

This cationic cholesterol derivative forms liposomes with L-α-phosphatidylethanolamine, dioleoyl, that efficiently transfect mammalian cell lines.

Properties

assay

~95%

storage temp.

−20°C

References

Reference

Gao, X. and Huang, L., A novel cationic liposome reagent for efficient transfection of mammalian cells. Biochem. Biophys. Res. Commun. 179, 280, (1991) abstract

Colosimo, A., et al., Gene transfection efficiency of tracheal epithelial cells by DC-Chol-DOPE/DNA complexes. Biochim. Biophys. Acta 1419, 186, (1999) abstract

Campbell, M.F., Lipofection reagents prepared by a simple ethanol injection technique. Biotechniques 18, 1027-1032, (1995) abstract

Caplen, N.J., et al., In vitro liposome-mediated DNA transfection of epithelial cell lines using the cationic liposome DC-Chol/DOPE. Gene Ther. 2, 603-613, (1995) abstract

Ajmani, P.S., and Hughes, J.A., 3β-[N-(N',N'-Dimethylaminoethane)carbamoyl]cholesterol (DC-Chol)-mediated gene delivery to primary rat neurons: characterization and mechanism. Neurochem. Res. 24, 699-703, (1999) abstract

Szala, S., et al., The use of cationic liposomes DC-Chol/DOPE and DDAB/DOPE for direct transfer of Escherichia coli cytosine deaminase gene into growing melanoma tumors. Gene Ther. 3, 1026-1031, (1996) abstract

Safety

WGK Germany