Monoclonal Anti-CD74 antibody produced in mouse 

nuclei from a SU-DHL-4 diffuse histiocytic human lymphoma cell line.

Recognizes the cell surface MHC class II-associated invariant chain molecule (II_i) expressed on B cells, some monocytes and interdigitating histiocytes. The antibody reacts with human mantle zone and germinal center B lymphocytes, interdigitating reticulum cells and small spindle shaped connective tissue macrophages in tonsils, lymph nodes and spleen and with medullary B cells and dendritic cells of the thymus. It also reacts with B cell lymphomas, Hodgkin's disease mononuclear cells, Reed-Sternberg cells and some chronic B lymphocytic leukemia cells. Tubercular granuloma giant cells and intertubular kidney cells, and focal reactivity with rare cases of lung squamous cell carcinoma, transitional cell carcinoma of the urinary bladder and a malignant melanoma cell line are also stained.¹ The staining pattern of lymphocytes was described as "perinuclear" or "cell membrane", while that of tissue macrophages was described as cytoplasmic. The antibody is reactive in immunoprecipitation (33, 35, 41 kDa), flow cytometry² and immunohistochemistry using frozen sections or B5-solution fixed or neutral buffered-formalin fixed, paraffin-embedded sections.
4th Workshop: code no. LN2
5th Workshop: code no. CD74.3

ascites fluid

LN-2

15 mM sodium azide

human

flow cytometry: suitable

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:200 using human tonsil sections

immunoprecipitation: suitable

IgG1

human ... CD74(972)

dry ice

−20°C

1. Epstein, A.L., et al. J. Immunol. 133, 1028, (1984) abstract

2. Yoshino, T., et al. Acta Med. Okayama 44, 243, (1990)

Cattoreti, G., et al. J. Pathol. 171, 83, (1993) abstract

Okon, E., et al. Cancer 56, 95, (1985) abstract

Marder, R.J., et al. Lab. Invest. 52, 497, (1985) abstract

, W. Knapp Workshop No. B129 Oxford , (1989)

Shin, S.S., et al. Hum. Pathol. 23, 686, (1992) abstract

Poston, N. and Hussain, I.F. J. Histochem. Cytochem. 41, 1503, (1993) abstract

Linder, J., R.A. DeLellis Advances in Immunohistochemistry New York, NY, USA , (1988), 277

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