C2969
Sigma

Monoclonal Anti-I-CAM-1 (CD54) antibody produced in mouse

clone 8.4A6, purified immunoglobulin, 2 mg/mL

Synonym

Anti-I-CAM-1

Monoclonal Anti-CD54

MDL number

MFCD00240668

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Descriptions

Antigen Background

CD54 (I-CAM-1) is a glycoprotein involved in mediation of intercellular adhesion. CD54 is expressed by activated endothelial cells and is also detected on cells of many other lineages and in plasma.

Immunogen

TNF-α activated human endothelial cells.

Specificity

The antibody recognizes the human CD54 (I-CAM-1) surface antigen by flow cytometry, ELISA, immunocytochemistry, and immunohistochemistry. It inhibits human leukocyte adhesion to activated endothelial cells and stimulates homotypic aggregation of SKW3 cells. The epitope recognized by the antibody is localized in domain D2 of the CD54 molecule.
5th Workshop: code no. S 100

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4.

Properties

antibody form

purified immunoglobulin

clone

8.4A6

sterility

0.2 μm filtered

mol wt

antigen mol wt 85-110 kDa

species reactivity