C3362
Sigma

Calcium-Binding Protein Protocols, Vol. 2: Methods and Techniques

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General description

Researchers review protein chemistry and behavior of this class of proteins, and provide experimental techniques for studying it both in vitro and in vivo. This second volume focuses on techniques for studying the solution structure, stability, dynamics, calcium-binding properties, and biological activity of calcium-binding proteins in general. In addition to enzymatic assays and more routine spectroscopic and protein chemistry techniques, there are also NMR approaches, thermodynamic analyses, kinetic measurements such as surface plasmon resonance, strategies for amino acid sequence alignments, and fluorescence methods to study the distribution of calcium and calcium-binding proteins in cells.

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Identifiers

ISBN-10

0-89603-689-8

ISBN-13

978-0-89603-689-5

Properties

publication info

H.J. Vogel, ed., Humana Press, 2002, 436 pp., hard cover

Table of Contents

Methods to Study Calcium-Binding Proteins Quantitative Analysis of Ca2+ Binding by Flow Dialysis
Calcium Binding to Proteins Studied via Competition with Chromophoric Chelators
Deconvolution of Calcium Binding Curves: Facts and Fiction
Absorption and Circular Dichroism Spectroscopy S
Fourier Transform Infrared Spectroscopy of Calcium-Binding Proteins
Steady State Fluorescence Spectroscopy
Fluorescence Methods for Measuring Calcium Affinity and Calcium Exchange with Proteins
Surface Plasmon Resonance of Calcium-Binding Proteins
Differential Scanning Calorimetry
Isothermal Titration Calorimetry
Multiangle Laser Light Scattering and Sedimentation Equilibrium
Small-Angle Solution Scattering Reveals Information on Conformational Dynamics in Calcium-Binding Proteins and in Their Interactions with Regulatory Targets
Investigation of Calcium-Binding Proteins Using Electrospray Ionization Mass Spectrometry
Synthetic Calcium-Binding Peptides Gary S. Shaw Proteolytic Fragments of Calcium-Binding Proteins
Electron Magnetic Resonance Studies of Calcium-Binding Proteins
Cadmium-113 and Lead-207 NMR of Calcium-Binding Proteins
Calcium-43 of NMR of Calcium-Binding Proteins
Exploring Familial Relationships Using Multiple Sequence Alignment
Structure Determination by NMR - Isotope Labeling
Protein Structure Calculation from NMR Data
Shape and Dynamics of Calcium Binding Proteins Investigated by Nitrogen-15 NMR Relaxation
The Use of Dipolar Couplings for the NMR Structure Refinement of a Pair of Calcium Binding EGF Domains
Vector Geometry Mapping: A Method to Characterise the Conformation of Helix-Loop-Helix Calcium Binding Proteins
Use of Calmodulin Antagonists and S-100 Protein Interacting Drugs for Affinity Chromatography
Enzymatic Assays to Compare Calmodulin Isoforms, Mutants and Chimeras
Gene Expression in Transfected Cells
Monitoring the Intracellular Free Ca2+-Calmodulin Concentration with Genetically-Encoded Fluorescent Indicator Proteins
Studying the Spatial Distribution of Ca2+-Binding Proteins: How Does it Work for Calmodulin?