Catalase from human erythrocytes 

Protein determined by biuret

Catalase activates the decomposition of hydrogen peroxide, a reactive oxygen species, into water and oxygen. It functions as a natural antioxidant, protecting cells against oxidative damage to proteins, lipids and nucleic acids. Catalase has also been used to study the role reactive oxygen species play in gene expression and apoptosis.

One unit will decompose 1.0 μmole of H₂O₂ per min at pH 7.0 at 25 °C, while the H₂O₂ conc. falls from 10.3 to 9.2 mM, measured by the rate of decrease of A₂₄₀.

Solution in 50 mM Tris, pH 8.0

≥90% (SDS-PAGE)

buffered aqueous solution

tetramer mol wt ~250 kDa

human ... CAT(847)

dry ice

−20°C

Sakata, N., et al., Oxidative damage of vascular smooth muscle cells by the glycated protein-cupric ion system. Atherosclerosis 136, 263-274, (1998)

Rossman, T.G., and Goncharova, E.I., Spontaneous mutagenesis in mammalian cells is caused mainly by oxidative events and can be blocked by antioxidants and metallothionein. Mutat. Res. 402, 103-110, (1998)

Aoshiba, K., et al., Role of p38-Mitogen-Activated Protein Kinase in Spontaneous Apoptosis of Human Neutrophils. J. Immunol. 162, 1692-1700, (1999)

Luo, Y., et al., Dopamine induces apoptosis through an oxidation-involved SAPK/JNK activation pathway. J. Biol. Chem. 273, 3756-3764, (1998)

Kaal, E.C., et al., Oxidant treatment causes a dose-dependent phenotype of apoptosis in cultured motoneurons. J. Neurosci. Res. 54, 778-786, (1998)

Yu, T.W., and Anderson, D., Reactive oxygen species-induced DNA damage and its modification: a chemical investigation. Mutat. Res. 379, 201-210, (1997)

Lapointe, S., et al., Binding of a bovine oviductal fluid catalase to mammalian spermatozoa. Biol. Reprod. 58, 747-753, (1998)

Yang, W.L., and Sun, A.Y., Paraquat-induced cell death in PC12 cells. Neurochem. Res. 23, 1387-1394, (1998)

1

FI4378000

A8056, 3-Amino-1,2,4-triazole