C3667
Sigma

Chondroitinase ABC from Proteus vulgaris

lyophilized powder, 50-250 units/mg protein (using chondroitin sulfate C as substrate)

Synonym

Chondroitin ABC Lyase

CAS Number

9024-13-9

Enzyme Commission (EC) Number

4.2.2.4 ( BRENDA | IUBMB )

MDL number

MFCD00130795

EG/EC Number

232-777-9

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Descriptions

Other Notes

Contains potassium phosphate buffer salts and stabilizer; BSA-free.

View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer

Packaging

Packages based on chondroitinase C activity.

Preparation Note

Affinity purified

Unit Definition

One unit will liberate 1.0 μmole of 2-acetamido-2-deoxy-3-O-(β-D-gluc-4-ene-pyranosyluronic acid)-4-O-sulfo-D-galactose from chondroitin sulfate A or 1.0 μmole of 2-acetamido-2-deoxy-3-O-(β-D-gluc-4-ene-pyranosyluronic acid)-6-O-sulfo-D-galactose from chondroitin sulfate C per min at pH 8.0 at 37 °C.

Properties

form

lyophilized powder

composition

Protein, ~10% Lowry

foreign activity

protease, essentially free

storage temp.

−20°C

References

Reference

Yamagata, T., et al. J. Biol. Chem. 243, 1523, (1968) abstract