C4608
Sigma

BID, Caspase-8-cleaved human

≥95% (SDS-PAGE), recombinant, expressed in Escherichia coli, buffered aqueous solution

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MDL number

MFCD03456215

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Descriptions

Analysis Note

Measured by its ability to induce cytochrome c release from isolated mouse liver mitochondria.

Biochem/physiol Actions

Caspase-8-cleaved BID relocates from the cytosol to the outer mitochondrial membrane where its interaction with Bak alters mitochondrial membrane permeability.

Physical form

0.2 μm filtered solution in 25 mM HEPES, pH 7.5, and 0.1 M KCl.

General description

Human BID is cleaved with caspase-8 to generate an amino-terminal fragment (amino acids 1-60, 7 kDa) and the C-terminal fragment (amino acids 61-195, 15 kDa). On size exclusion chromatography cleaved BID elutes as a single band corresponding to 27 kDa indicating that the two fragments remain associated.