| C5224 Sigma |
Chromatin: A Practical Approach |
General description
This title provides clear explanations of a wide range of techniques from the initial isolation and fractionation of chromatin and linker histones, to the in vitro assembly of chromatin and nuclear structures in Xenopus egg extracts.
Table of Contents
1. Isolation and fractionation of chromatin and linker histones
2. Extraction and separation of core histones and non-histone chromosomal proteins
3. Mapping chromatin structure in Drosophila
4. In vivo footprint and chromatin analysis by LMPCR
5. Analysis of nuclear scaffold attachment regions
6. Electron microscopy of chromatin
7. Counting nucleosome cores on circular DNA using topoisomerase I
8. In vitro reconstitution of chromatin and analysis of nucleosome positioning
9. Transcriptional and structural analysis of chromatin assembled in vivo
10. An assembly of chromatin and nuclear structures in Xenopus egg extracts
Appendix: List of Suppliers
Index
2. Extraction and separation of core histones and non-histone chromosomal proteins
3. Mapping chromatin structure in Drosophila
4. In vivo footprint and chromatin analysis by LMPCR
5. Analysis of nuclear scaffold attachment regions
6. Electron microscopy of chromatin
7. Counting nucleosome cores on circular DNA using topoisomerase I
8. In vitro reconstitution of chromatin and analysis of nucleosome positioning
9. Transcriptional and structural analysis of chromatin assembled in vivo
10. An assembly of chromatin and nuclear structures in Xenopus egg extracts
Appendix: List of Suppliers
Index
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