C5348
Sigma

Anti-Corticotropin Releasing Factor antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

Synonym

Anti-CRF

MDL number

MFCD02096145

Descriptions

Immunogen

synthetic peptide corresponding to the C-terminal of human CRF (amino acids 25-41 with N-terminally added lysine) conjugated to KLH.

Specificity

The antibody does not cross-react with human or rat CRF (6-33), sheep CRF, rat urocortin, PACAP38, human ACTH, human ACTH (18-39) and sauvagine.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Properties

antibody form

IgG fraction of antiserum

form

buffered aqueous solution

species reactivity

human, rat, mouse, pig, canine, frog, sheep, bovine

application(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100 using microwave-treated sections of human placental tissue

microarray: suitable

radioimmunoassay: 1:10,000 using 2-¹²⁵I-His¹²-CRF, human

Gene Information

mouse ... Crh(12918)

human ... CRH(1392)

rat ... Crh(81648)

shipped in

dry ice

storage temp.

−20°C

Safety

WGK Germany