Monoclonal Anti-Biotin–Cy3 antibody produced in mouse 

Biotin-KLH conjugate

The antibody recognizes free biotin and biocytin using competitive ELISA. Using indirect ELISA or immuno­histo­chemical staining, the antibody also recognizes biotin conjugated to various immunoglobulins. Specificity is evaluated using biotinylated goat antibodies to human and rabbit antigens coated on multiwell plates. The antibody may be used in amplification techniques, ELISA, immunoblotting, in situ nucleic acid hybridization, flow cytometry, image analysis or confocal microscopy.

Solution in phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

In some applications, localization of biotinylated probes with avidin produces high background levels. Anti-biotin reagents may be substituted for avidin to decrease non-specific binding.

Cy3 is a trademark of GE Healthcare.

Cy is distributed under license from Amersham Biosciences Limited.

purified immunoglobulin

BN-34

buffered aqueous solution

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:400

indirect immunofluorescence: 1:100

IgG1

wet ice

2-8°C

Childs, G.V., Simultaneous identification of a specific gene protein product and transcript using combined immunocytochemistry and in situ hybridization with non-radioactive probes. Scanning Microsc. 10, 17-24, (1996)

Elborough, K.M., et al., Biotin carboxyl carrier protein and carboxyltransferase subunits of the multi-subunit form of acetyl-CoA carboxylase from Brassica napus. Biochem. J. 315, 103-112, (1996)

Cooper, K.M., et al,, An immunohistochemical study of the distribution of biotin in tissues of pigs and chickens. Res. Vet. Sci. 63, 219-225, (1997)

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