Monoclonal Anti-Actin–Cy3 antibody produced in mouse 

synthetic actin C-terminal peptide Ser-Gly-Pro-Ser-Ile-Val-His-Arg-Lys-Cys-Phe, attached to a Multiple Antigen Peptide (MAP) backbone.

To view an Actin antibody selection guide, please click here.

The antibody recognizes an epitope located on the C-terminal end of actin conserved in all actin isoforms. It specifically labels actin in a wide variety of tissues and species. The epitope recognized by the antibody is resistant to formalin-fixation and paraffin-embedding. Zinc-formalin, B5, ethanol, methacarn, Brunnel's or Bouin's solutions may also be used as fixatives.

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Distributed under license from Amersham Biosciences Limited.

Cy3 is a trademark of GE Healthcare.

purified immunoglobulin

AC-40

buffered aqueous solution

antigen mol wt 42 kDa

human, bovine, sheep, goat, pig, rabbit, canine, mouse, rat, guinea pig, hamster, chicken, carp, viper, Xenopus, snail

direct immunofluorescence: 1:75 using cultured human or chicken fibroblasts

IgG2a

human ... ACTA1(58), ACTA2(59), ACTB(60), ACTC1(70), ACTG1(71), ACTG2(72)

mouse ... Acta1(11459), Acta2(11475), Actb(11461), Actc1(11464), Actg1(11465), Actg2(11468)

rat ... Acta1(29437), Acta2(81633), Actb(81822), Actc1(29275), Actg1(287876), Actg2(25365)

wet ice

2-8°C

Albiges-Rizo, C., Down regulation of talin alters cell adhesion and processing of the α5β1 integrin. J. Cell Sci. 108, 3317, (1995)

NWG