C6198
Sigma

Monoclonal Anti-Actin, α-Smooth Muscle-Cy3^™ antibody produced in mouse

clone 1A4, purified immunoglobulin, buffered aqueous solution

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Synonym

Monoclonal Anti-Actin, α-Smooth Muscle

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Descriptions

Immunogen

N-terminal synthetic decapeptide of α-smooth muscle actin.

Other Notes

To view an Actin antibody selection guide, please click here.

Specificity

The antibody (also known as anti-α-Sm-1) is specific for the single isoform of α-smooth muscle actin. It reacts specifically with α-smooth muscle actin in immunoblotting assays and labels smooth muscle cells in frozen or formalin-fixed, paraffin-embedded tissue sections.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide, as a preservative.

Legal Information

Cy is distributed under license from Amersham Biosciences Limited.

Cy3 is a trademark of GE Healthcare.

Properties

antibody form

purified immunoglobulin

clone

1A4

form

buffered aqueous solution

mol wt

antigen mol wt ~42 kDa

species reactivity

human, mouse, rat, bovine, chicken, frog, goat, guinea pig, rabbit, canine, sheep, snake

application(s)

immunohistochemistry (frozen sections): 1:200 using human tonsil or appendix

isotype

IgG2a

Gene Information

human ... ACTA2(59)

mouse ... Acta2(11475)

rat ... Acta2(81633)

shipped in

wet ice

storage temp.

2-8°C

References

Reference

Barnes, V.L., et al. J. Histochem. Cytochem. 47, 787, (1999) abstract

Skalli, O., et al., A monoclonal antibody against α-smooth muscle actin: a new probe for smooth muscle differentiation. J. Cell Biol. 103, 2787, (1986)

Safety

WGK Germany