C6286
Sigma

Cathepsin B from bovine spleen

lyophilized powder, ≥10 units/mg protein

Synonym

Cathepsin B₁

CAS Number

9047-22-7

Enzyme Commission (EC) Number

3.4.22.1 ( BRENDA | IUBMB )

MDL number

MFCD02662345

Expand/Collapse All

Descriptions

Unit Definition

One unit will hydrolyze 1 μmole of Z-lysine p-nitrophenyl ester per min at pH 5.0 at 25 °C.

Physical form

Lyophilized powder containing sodium phosphate, sodium chloride and ~6% EDTA as stabilizer.

Biochem/physiol Actions

Cathepsin B has been found to cleave procaspase 1 and procaspase 11 and to induce apoptosis in digitonin-permeabilized cells. Translocation of cathepsin B from the cytoplasm to the nucleus contributes to bile salt induced apoptosis of rat hepatocytes. Levels of cathepsin B in PC12 cells significantly decrease 12 to 24 hours after apoptosis is induced.

Properties

form

lyophilized powder

composition

Protein, ≥30% biuret

Gene Information

cow ... CTSB(281105)

storage temp.

−20°C

References

Reference

Vancompernolle, K., et al., Atractyloside-induced release of cathepsin B, a protease with caspase-processing activity. FEBS Lett. 438, 150-158, (1998)

Shibata, M., et al., Participation of cathepsins B and D in apoptosis of PC12 cells following serum deprivation. Biochem. Biophys. Res. Commun. 251, 199-203, (1998)

Ohsawa, Y., et al., An ultrastructural and immunohistochemical study of PC12 cells during apoptosis induced by serum deprivation with special reference to autophagy and lysosomal cathepsins. Arch. Histol. Cytol. 61, 395-403, (1998)

Barrett, A.J. and Kirschke, H., Cathepsin B, cathepsin H and cathepsin L. Meth. Enzymol. 80, 535-561, (1981)

Roberts, L.R., et al., Cathepsin B contributes to bile salt-induced apoptosis of rat hepatocytes. Gastroenterology 113, 1714-1726, (1997)

Safety

WGK Germany

10-21