C7104
Sigma

Monoclonal Anti-Cdc27 antibody produced in mouse

~2 mg/mL, clone AF3.1, purified immunoglobulin, buffered aqueous solution

MDL number

MFCD01633250

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Descriptions

Immunogen

synthetic peptide corresponding to the C-terminus 10 residues of human Cdc27.

Properties

antibody form

purified immunoglobulin

clone

AF3.1

form

buffered aqueous solution

mol wt

antigen mol wt 97 kDa

species reactivity

human, bovine, canine, rat, mouse, Xenopus

concentration

~2 mg/mL

application(s)

immunoblotting: 1-2 μg/mL using a HeLa Cell nuclear extract

immunocytochemistry: suitable using 4% paraformaldehyde fixation, Triton X-100 permeabilization

immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable

immunoprecipitation: suitable

indirect ELISA: suitable

microarray: suitable

isotype

IgG2b

Gene Information

mouse ... Cdc27(217232)

rat ... Cdc27(360643)

human ... CDC27(996)

shipped in

dry ice

storage temp.

−20°C

References

Reference

Yamano, H., et al., The role of the destruction box and its neighbouring lysine residues in cyclin B for anaphase ubiquitin-dependent proteolysis in fission yeast: defining the D-box receptor. EMBO J. 17, 5670-5678, (1998) abstract

Gieffers, C., et al., Expression of the CDH1-associated form of the anaphase-promoting complex in postmitotic neurons. Proc. Natl. Acad. Sci. USA 96, 11317-11322, (1999) abstract

Safety

WGK Germany