Concanavalin A from Canavalia ensiformis (Jack bean) 

Where reported, agglutination activity is expressed in μg/ml and is determined from serial dilutions in phosphate buffered saline, pH 6.8, containing Ca²⁺ and Mn²⁺ of a 1 mg per mL solution. This activity is the lowest concentration to agglutinate a 2% suspension of human erythrocytes after 1 hr incubation at 25 °C.

Used for the purification of glycoproteins containing mannose

Con A is not blood group specific but has an affinity for terminal α-D-mannosyl and α-D-glucosyl residues. Ca²⁺ and Mn²⁺ ions are required for activity. Con A dissociates into dimers at pH 5.6 or below. Between pH 5.8 and pH 7.0, Con A exists as a tetramer; above pH 7.0 higher aggregates are formed. Con A exhibits mitogenic activity which is dependent on its degree of aggregation. Succinylation results in an active dimeric form which remains a dimer above pH 5.6.

Suspension in 1.0 M NaCl + 0.1 M potassium phosphate, pH 6.0, with 1 mM CaCl₂, 1 mM MgCl₂ and 1 mM MnCl₂ containing 0.02% thimerosal

Sepharose is a registered trademark of GE Healthcare.

Type VI

saline suspension

15-30 mg concanavalin A protein per mL

cross-linked 4% beaded agarose

cyanogen bromide

amino

1 atom

5-15 mg/mL binding capacity (yeast mannan)(in 0.01 M phosphate buffered saline, pH 6.8, containing 1 mM each CaCl₂, MgCl₂ and MnCl₂)

agarose conjugate

2-8°C

Lotan, R Biochemistry 16, 1787-1794, (1977) abstract

Reeke, G.N., et al. Ann. N.Y. Acad. Sci. 234, 369, (1974)

, Bittiges, H. and Schnebli, H.P, ed. Concanavalin A as a Tool London , (1976)

Gunther, G.R., et al. Proc. Natl. Acad. Sci. USA 70, 1012, (1973)

Kalb, A.J. and Lustig, A. Biochim. Biophys. Acta 168, 366, (1968)

Kobata, A., and Endo, T. J. Chromatogr. 597, 111-122, (1992) abstract

Merck 13,2521

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