C7559
Sigma

Chloroform

≥99%, PCR Reagent, contains amylenes as stabilizer

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Synonym

Methylidyne trichloride

Trichloromethane

Molecular Formula

CHCl₃

Molecular Weight

119.38

CAS Number

67-66-3

Beilstein Registry Number

1731042

MDL number

MFCD00000826

PubChem Substance ID

24892970

EG/EC Number

200-663-8

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Descriptions

Application

Facilitates recovery of the aqueous phase of PCRs which have been overlaid with mineral oil.

Properties

grade

PCR Reagent

vapor density

4.1 (vs air)

vapor pressure

160 mmHg ( 20 °C)

assay

≥99%

contains

amylenes as stabilizer

packaging

vial of 1.5 mL

refractive index

n20/D 1.445(lit.)

60.5-61.5 °C(lit.)

−63 °C(lit.)

density

1.492 g/mL at 25 °C(lit.)

foreign activity

DNase, RNase, none detected

References

Merck

Merck 13,2160

Beilstein

Beil. 1,IV,42

Fieser

Fieser 1,29 / Fieser 1,30 / Fieser 1,130 / Fieser 3,140 / Fieser 4,22 / Fieser 5,28 / Fieser 8,92 / Fieser 12,517 / Fieser 14,167 / Fieser 15,84

reference

Aldrich MSDS 1, 405:C / Corp MSDS 1 (1), 786:C / FT-IR 2 (1), 117:D / FT-IR 1 (1), 83:A / FT-NMR 1 (1), 122:A / IR-Spectra (3), 51:G / IR-Spectra (2), 49:E / NMR-Reference 2 (1), 81:C / RegBook 1 (1), 81:D / Sax 6, 722 / Structure Index 1, 9:D:10 / Vapor Phase 3, 116:C

Safety

Hazard Codes

Risk Statements

22-38-40-48/20/22

Safety Statements

36/37

RIDADR

UN1888 6.1 /PG 3

WGK Germany

RTECS

FS9100000