C7609
Sigma

Cdc25B, active human

~30%, recombinant, expressed in Escherichia coli, buffered aqueous glycerol solution, ~3,000 units/mg

MDL number

MFCD02687018

Descriptions

Other Notes

Full-length human Cdc25B with a glutathione S-transferase (GST) tag at the amino terminus.

Preparation Note

Purified using GST-Agarose.

Unit Definition

One unit of cdc25 B activity is equal to 1 pmole phosphate hydrolyzed from 3-o-methylfluorescein phosphate (OMFP) per minute.

Physical form

Solution in 50 mM Tris-HCl pH 8.0, 150 mM NaCl, 25 mM glutathione, 5 mM dithiothreitol and 25% glycerol.

Properties

recombinant

expressed in Escherichia coli

assay

~30%

form

buffered aqueous glycerol solution

mol wt

mol wt ~92 kDa

storage temp.

−20°C

References

Reference

Fauman, E.B., et al. Cell 93, 617, (1998)

Gottlin, E., et al. J. Biol. Chem. 271, 27445, (1996)

Safety

WGK Germany

Related Categories

... Apoptosis and Cell Cycle > Cyclins and Nuclear Proteins

... Protein Kinases and Phosphatases > Protein Phosphatases

... Cell Signaling Enzymes > Signal Transduction

MSDS

Certificate of Analysis

Related Categories

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C7609 (Sigma)